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Image Search Results
Journal: Autophagy
Article Title: Inhibition of autophagy and induction of glioblastoma cell death by NEO214, a perillyl alcohol-rolipram conjugate.
doi: 10.1080/15548627.2023.2242696
Figure Lengend Snippet: Figure 3. NEO214 has an adverse effect on lysosomal function and blocked autophagic flux in glioma cells dependent on MTOR signaling. (A-B) Expression analysis of the autophagy-lysosome relevant genes in 100 μmol/L NEO214-treated U251 and T98G cells. (C) WB analysis of ATP6V1G1, ATP6V1D, ATP6V1H and ATP6V1C1 in U251 and T98G cells were treated with NEO214(100 μmol/L) in full-medium 24 h. (D) NEO214 does not affect the autophagosome formation process in glioma cells. Western blot analysis of the protein expression of BECN1, ATG3, ATG5, ATG9A, LC3, and SQSTM1 in U251 and T98G cells treated by 100 μmol/L NEO214 for 24 h. (E) Western blot analysis of MTOR signaling proteins expressions in U251 and T98G cells treated by 100 μmol/L NEO214 24 h. (n = 3; *, P < 0.05; **, P < 0.01; ***, P < 0.001).
Article Snippet: Immunoblotting was performed with PARP (Cell Signaling Technology, CS9542), LC3B (Cell Signaling Technology, CS-2775), SQSTM1/ p62 (Cell Signaling Technology, CS-5114), BECN1/beclin 1 (Cell Signaling Technology, CS-3738), RPTOR/raptor (Cell Signaling Technology, CS-48648), RICTOR (Cell Signaling Technology, CS-2140), p-AKT (Cell Signaling Technology, CS-4058), p-RPS6KB/p70S6K (Cell Signaling Technology, CS-9206), RPS6KB/p70S6K (Cell Signaling Technology, CS9202), p-MTOR (Cell Signaling Technology, CS-2971), p-EIF4EBP1 (Cell Signaling Technology, CS-9456), EIF4EBP1 (Cell Signaling Technology, CS-9452), MGMT (Cell Signaling Technology, CS-2739S), CTSD/cathepsin D (Cell Signaling Technology, CS-2284), ATG3 (Cell Signaling Technology, CS-3415), ATG5 (Cell Signaling Technology, CS-12994), TP53 (Cell Signaling Technology, CS-9282), cleaved CASP7/caspase 7 (Cell Signaling Technology, CS-9491), TUBA1B/α-Tubulin (Cell Signaling Technology, CS-2125), LMNB1/Lamin B1 (Cell Signaling Technology, CS-13435), ATP6V1A (Abcam, ab -199,326), ATP6V1B2 (Abcam, ab -73,404), MTOR (Abcam, ab-2732), and CTSB/cathepsin B (Abcam, ab58802), TFEB (ThermoFisher Scientific, PAS-34360; 1:100 for IP, 1:200 for IF, 1:1000 for WB), TSC2 (Proteintech, 24601–1-AP), TSC1 (Proteintech, 20988–1-AP), ATP6V1D (Proteintech, 14920– 1-AP),
Techniques: Expressing, Western Blot